Buffers and Culture media

Tris Buffer

August 12, 2019 / 0 Comments

Introduction: Tris (hydroxymethyl) aminomethane is also known as THAM, with pKa 8.06. Tris (M.W: 121.14) is a white crystalline powder having molecular formula C4H11NO3. It forms a clear solution in water. Tris is used as a buffering system in most of the biological experiments. Applications: Tris is a component of: DNA extraction buffer (Edwards buffer)…

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TE Buffer

August 10, 2019 / 0 Comments

TE buffer (a.k.a Tris EDTA buffer) is one of the extensively used buffers in preparation and storage of DNA and RNA protocols. TE buffer is also known as T10E1 Buffer due to their ratio in the working concentration, which is 10mM and 1 mM respectively. As the name suggests, the TE buffer is composed of…

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Arabidopsis Pollen Germination Medium (Li H 1999)

May 26, 2019 / 0 Comments

The pollen germination medium (PGM) is used for allowing the pollen to germinate either by in vitro or semi-in vitro methods. This web page deals with the composition and preparation of PGM as mentioned in the article titled “Control of Pollen Tube Tip Growth by a Rop GTPase–Dependent Pathway That Leads to Tip-Localized Calcium Influx”…

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TAE buffer (Tris-acetate-EDTA)

April 14, 2019 / 0 Comments

Tris-acetate-EDTA (TAE) is one of the most commonly used buffers for DNA and RNA Agarose electrophoresis. It has a lower buffering capacity compared to TBE (Tris-borate-EDTA) but runs nucleic acids faster, hence became the first choice. The composition that is currently being used is developed by the contribution of different research groups in the early…

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LB broth / LB medium

January 14, 2019 / 0 Comments

Giuseppe Bertani formulated LB broth in 1951 in an attempt to optimise Shigella growth and plaque formation. Although LB media is known as “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”, it was initially named as “Lysogeny Broth”. LB broth’s agar form should be designated LA but now referring as LBA/LB. There are many formulations available…

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PBST Buffer (PBS with Tween 20)

January 2, 2019 / 0 Comments

PBST or PBS-T (phosphate buffered saline with Tween 20) is PBS buffer with detergent such as Tween 20 or Triton X-100 (CSH Protocols recommends Tween 20). Detergent concentration can vary depends on requirement but generally vary from 0.05 – 2% (CSHP recommends 0.05). We are giving composition based on CSHL protocols in the tabular form.…

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4% Paraformaldehyde (PFA) solution preparation

January 1, 2019 / 0 Comments

Paraformaldehyde (PFA) in PBS is one of the widely used fixatives for Immuno-histochemistry (IHC) and fluorescent protein labelled samples. Paraformaldehyde is a polymer of formaldehyde with a wide range of monomeric units typically 8-100. PFA does not have the capacity to fix samples, hence it must be depolymerised in the solution. Heating the PFA powder…

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Phosphate Buffered Saline (PBS)

December 21, 2018 / 0 Comments

Phosphate buffered saline (a.k.a PBS or PBS buffer) is one of the most used buffers since it is isotonic to most of the cells. We have placed reagents required for preparation in a table. This table also contains information on molarity of each reagent and amount of salt (s) to be added for making 500mL…

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Arabidopsis Pollen Germination Medium (Leonor 2007)

December 5, 2018 / 0 Comments

There are a handful of Pollen Germination Media (PGM) formulated for one of the widely used model plant, Arabidopsis. In this article, we have provided composition of one of the robust PGM. This PGM composition is adopted from a publication by Leonor 2007 (see the reference at the end of the article). If you are…

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