Fluorescent Dyes Excitation and Emission

Caution: Excitation max and Emission max of the fluorescent molecules are subjected to the microenvironment. This results in a variation in the values between one source to another.

Hence, the values to be taken as guidelines. Some dyes like DAPI, PI, etc.. differ in the spectra after binding to the target molecules. For such dyes, we have provided the Ex and Em when they are bound to their targets.

Dye Full name Excitation max Emission max
DAPI 4′,6-Diamidino-2-phenylindole 358 461
PI Propidium iodide 535 617
A488 Alexa 488 498 519
FITC Fluorescein isothiocyanate 497 515
A350 Alexa Fluor 350 346 442
A405 Alexa Fluor 405 401 421
A532 Alexa Fluor 532 532 554
A546 Alexa Fluor 546 556 573
A555 Alexa Fluor 555 555 580
A568 Alexa Fluor 568 578 603
A594 Alexa Fluor 594 590 617
A647 Alexa Fluor 647 650 665
A680 Alexa Fluor 680 679 702
A700 Alexa Fluor 700 702 723
A750 Alexa Fluor 750 749 775
Coumarin 387 470
Cy3 Cyanine3 554 568
Cy5 Cyanine5 649 666
Oregon Green 488 501 526
Pacific Blue 410 455
Pacific Green 411 510
Pacific Orange 400 551
PerCP-Cyanine5.5 Peridinin chlorophyll protein-Cyanine5.5 482 690
TRITC Tetramethylrhodamine 557 576
Texas Red 596 615
eFluor 450 405 450
PE-Cyanine7 565 774
eFluor 506 405 506
eFluor 660 633 669
PerCP-eFluor 710 633 710
APC-eFluor 780 655 780
Super Bright 436 414 436
Super Bright 600 414 600
Super Bright 645 414 645
Super Bright 702 414 702
Super Bright 780 414 780
SYTO 9 483 503
SYTOX Green 504 523
TO-PRO-3 642 661