Fluorescent Dyes Excitation and Emission

Note: The fluorescent molecules’ Excitation and Emission max are subjected to the microenvironment. This results in a variation in the values between one source to another.

Hence, the values are to be taken as guidelines. Some dyes, like DAPI, PI, etc.. differ in the spectra after binding to the target molecules. We have provided the Ex and Em for such dyes when they are bound to their targets.