Fluorescent Dyes Excitation and Emission

Note: Excitation max and Emission max of the fluorescent molecules are subjected to the microenvironment. This results in a variation in the values between one source to another.

Hence, the values are to be taken as guidelines. Some dyes like DAPI, PI, etc.. differ in the spectra after binding to the target molecules. For such dyes, we have provided the Ex and Em when they are bound to their targets.