Buffers and Solutions

Paraformaldehyde (PFA) is widely used fixative for IHC and fluorescent protein samples. in this post, you learn preparation of PFA…

Table Of Contents Introduction The authorship is often contributed to Gomori (1955) but Gomori is in this publication citing an…

The Laemmli sample buffer or Laemmli buffer is used for loading and better resolving of SDS-PAGE gels. In this article,…

PBS-T is washing buffer used in many methods. Here you will learn the composition and uses of PBS-T buffer.

Composition and preparation of Phosphate Buffered Saline (PBS) is explained in step-by-step. PBS is used as base component for many…

Ponceau S staining solution preparation, staining and destaining procedures were discussed in detail.

In this article, you will learn preparation of 0.1 to 0.5M EDTA step by step. EDTA is a widely used…

Table Of Contents Introduction Various authors (Gomori, 1955; Pearse 1980) are cited as a source for buffer composition, although it…

TAE buffer is used in electrophoresis. In this post you learn preparation of TAE buffer step-by-step.

The TBE buffer (Tris Borate EDTA buffer) is used in DNA/RNA electrophoresis. In this article, you learn how to prepare…