Buffers and Solutions

4% Paraformaldehyde (PFA) solution preparation

Paraformaldehyde (PFA) is widely used fixative for IHC and fluorescent protein samples. in this post, you learn preparation of PFA…

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Citrate Buffer recipe

Table Of Contents Introduction The authorship is often contributed to Gomori (1955) but Gomori is in this publication citing an…

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Laemmli buffer: Preparation (1x,2x & 4x) and principle

The Laemmli sample buffer or Laemmli buffer is used for loading and better resolving of SDS-PAGE gels. In this article,…

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PBST Buffer (PBS with Tween 20)

PBS-T is washing buffer used in many methods. Here you will learn the composition and uses of PBS-T buffer.

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Phosphate Buffered Saline (PBS)

Composition and preparation of Phosphate Buffered Saline (PBS) is explained in step-by-step. PBS is used as base component for many…

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Ponceau S staining (preparation and protocol)

Ponceau S staining solution preparation, staining and destaining procedures were discussed in detail.

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Preparation of EDTA solution

In this article, you will learn preparation of 0.1 to 0.5M EDTA step by step. EDTA is a widely used…

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Sodium acetate buffer

Table Of Contents Introduction Various authors (Gomori, 1955; Pearse 1980) are cited as a source for buffer composition, although it…

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TAE buffer (Tris-acetate-EDTA)

TAE buffer is used in electrophoresis. In this post you learn preparation of TAE buffer step-by-step.

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TBE buffer (Tris-Borate-EDTA buffer) 1x, 5x & 10x

The TBE buffer (Tris Borate EDTA buffer) is used in DNA/RNA electrophoresis. In this article, you learn how to prepare…

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