Cloning methods
Primer designing is the first and one of the critical steps for successful cloning. In this article, we have demonstrated the primer designing for various cloning methods.
Read MoreTOPO TA cloning is a better alternative to the conventional (ligation-based) TA cloning method. This article discusses the principle, advantages, and limitations of TOPO TA cloning.
Read MoreDirectional TOPO cloning is an easy sub-cloning method to clone DNA fragments without ligase. This article discusses the principle, advantages, and limitations.
Read MoreBlunt-end TOPO cloning is a better alternative to the conventional (ligation-based) cloning method. This article discusses the principle, advantages, and limitations of blunt-end TOPO cloning.
Read MoreTA cloning is a universal cloning method. In this article, you will learn history, applications, advantages, limitations, and cloning procedure in detail.
Read MoreGibson assembly is one of the most used methods for seamless cloning. In this article, you will learn history, applications, advantages, limitations, and the cloning procedure in detail.
Read MoreIn-Fusion cloning is one of the most used methods for seamless cloning. In this article, you will learn history, applications, advantages, limitations, and the cloning procedure in detail.
Read MoreBlunt-end cloning is a universal cloning method. In this article, you will learn history, applications, advantages, limitations, and cloning procedure in detail.
Read MoreGibson assembly and In-Fusion are the two most used cloning methods for scar-free cloning of multiple DNA fragments. This article explains the differences between the two cloning methods.
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