Cloning methods

Primer designing is the first and one of the critical steps for successful cloning. In this article, we have demonstrated the primer designing for various cloning methods.

TOPO TA cloning is a better alternative to the conventional (ligation-based) TA cloning method. This article discusses the principle, advantages, and limitations of TOPO TA cloning.

Directional TOPO cloning is an easy sub-cloning method to clone DNA fragments without ligase. This article discusses the principle, advantages, and limitations.

Blunt-end TOPO cloning is a better alternative to the conventional (ligation-based) cloning method. This article discusses the principle, advantages, and limitations of blunt-end TOPO cloning.

TA cloning is a universal cloning method. In this article, you will learn history, applications, advantages, limitations, and cloning procedure in detail.

Gibson assembly is one of the most used methods for seamless cloning. In this article, you will learn history, applications, advantages, limitations, and the cloning procedure in detail.

In-Fusion cloning is one of the most used methods for seamless cloning. In this article, you will learn history, applications, advantages, limitations, and the cloning procedure in detail.

Blunt-end cloning is a universal cloning method. In this article, you will learn history, applications, advantages, limitations, and cloning procedure in detail.

Gibson assembly and In-Fusion are the two most used cloning methods for scar-free cloning of multiple DNA fragments. This article explains the differences between the two cloning methods.