Cloning methods

Blunt end cloning hero image showing an overview of steps involved in the cloning

Blunt-end cloning: Ultimate guide

Blunt-end cloning is a universal cloning method. In this article, you will learn history, applications, advantages, limitations, and cloning procedure…

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Blunt-end TOPO Cloning

Blunt-end TOPO cloning is a better alternative to the conventional (ligation-based) cloning method. This article discusses the principle, advantages, and…

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Directional TOPO Cloning

Directional TOPO cloning is an easy sub-cloning method to clone DNA fragments without ligase. This article discusses the principle, advantages,…

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Gibson assembly

Gibson assembly is one of the most used methods for seamless cloning. In this article, you will learn history, applications,…

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Gibson assembly vs. In-Fusion cloning

Gibson assembly and In-Fusion are the two most used cloning methods for scar-free cloning of multiple DNA fragments. This article…

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In-Fusion Cloning

In-Fusion cloning is one of the most used methods for seamless cloning. In this article, you will learn history, applications,…

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Primer Designing – Demonstration step by step

Primer designing is the first and one of the critical steps for successful cloning. In this article, we have demonstrated…

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TA cloning: Ultimate guide

TA cloning is a universal cloning method. In this article, you will learn history, applications, advantages, limitations, and cloning procedure…

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TOPO TA Cloning

TOPO TA cloning is a better alternative to the conventional (ligation-based) TA cloning method. This article discusses the principle, advantages,…

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