Fluorescent Protein Properties
Excitation maxima
Emission maxima
Brightness
pKa
Bleaching time
Maturation time
Ex max
430
Em max
474
pKa
3.3
Brightness
23.1
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
7.5
Ex 395:
43.2
Ex 405:
60.7
Ex 458:
67
Ex max
395
Em max
509
pKa
4.5
Brightness
19.7
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
39.9
Ex 395:
99.9
Ex 405:
91.5
Ex 458:
26
Ex 488:
23.1
Ex max
433
Em max
475
pKa
4.7
Brightness
26.7
Aggregation
Maturation
Bleaching
36
Percentage of excitation at
Ex 355:
6.9
Ex 395:
33.9
Ex 405:
50.9
Ex 458:
90.8
Ex 488:
5.4
Ex max
456
Em max
480
pKa
Brightness
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
56.3
Ex 395:
47.5
Ex 405:
57.4
Ex 458:
99.8
Ex max
516
Em max
529
pKa
5.7
Brightness
58.5
Aggregation
Maturation
Bleaching
49
Percentage of excitation at
Ex 458:
8
Ex 488:
35.1
Ex 514:
99
Ex 532:
15
Ex max
505
Em max
515
pKa
6.2
Brightness
84.4
Aggregation
Maturation
30
Bleaching
50
Percentage of excitation at
Ex 458:
22.5
Ex 488:
59.1
Ex 514:
63.3
Ex max
497
Em max
589
pKa
5.5
Brightness
30.4
Aggregation
Maturation
15
Bleaching
111
Percentage of excitation at
Ex 395:
6.5
Ex 405:
9.8
Ex 458:
60.7
Ex 488:
95.6
Ex 514:
98.7
Ex 532:
86.8
Ex 543:
59.1
Ex 555:
26.8
Ex 561:
17.7
Ex max
435
Em max
477
pKa
5.02
Brightness
17.8
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
6.9
Ex 395:
33.9
Ex 405:
50.9
Ex 458:
90.8
Ex 488:
5.4
Ex max
558
Em max
583
pKa
Brightness
49.3
Aggregation
Maturation
1600
Bleaching
Percentage of excitation at
Ex 405:
6.7
Ex 458:
36.1
Ex 488:
46.7
Ex 514:
68.6
Ex 532:
57.7
Ex 543:
99.9
Ex 555:
85.8
Ex 561:
60.8