Fluorescent Protein Properties
Excitation maxima
Emission maxima
Brightness
pKa
Bleaching time
Maturation time
Ex max
433
Em max
475
pKa
3.2
Brightness
34.8
Aggregation
Maturation
69.8
Bleaching
Percentage of excitation at
Ex 395:
39.6
Ex 405:
57.6
Ex 458:
82.7
Ex max
587
Em max
610
pKa
4.5
Brightness
15.8
Aggregation
Maturation
15
Bleaching
68
Percentage of excitation at
Ex 355:
13.2
Ex 488:
7.6
Ex 514:
20.9
Ex 532:
40
Ex 543:
53.5
Ex 555:
59.9
Ex 561:
63.9
Ex 594:
86.7
Ex 612:
20.9
Ex max
589
Em max
610
pKa
3.3
Brightness
17.5
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 488:
6.8
Ex 514:
18.7
Ex 532:
37.5
Ex 543:
48.9
Ex 555:
55.5
Ex 561:
64.1
Ex 594:
90.5
Ex 612:
28.6
Ex max
516
Em max
529
pKa
Brightness
69.56
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 458:
8
Ex 488:
35.4
Ex 514:
99
Ex 532:
15
Ex max
506
Em max
518
pKa
6.5
Brightness
85
Aggregation
Maturation
43.5
Bleaching
80
Percentage of excitation at
Ex 458:
22.5
Ex 488:
59.1
Ex 514:
63.3
Ex max
433
Em max
475
pKa
Brightness
13.5
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
6.9
Ex 395:
33.9
Ex 405:
50.9
Ex 458:
90.8
Ex 488:
5.4
Ex max
488
Em max
507
pKa
6
Brightness
33.6
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
5.6
Ex 395:
16
Ex 405:
17.3
Ex 458:
61.2
Ex 488:
99.8
Ex 514:
14.7
Ex max
487
Em max
509
pKa
6
Brightness
39.1
Aggregation
Maturation
11.2
Bleaching
101
Percentage of excitation at
Ex 355:
5.6
Ex 395:
16
Ex 405:
17.3
Ex 458:
61.2
Ex 488:
99.8
Ex 514:
14.7
Ex max
487
Em max
562
pKa
4
Brightness
2
Aggregation
Maturation
Bleaching
5.9
Percentage of excitation at
Ex 395:
8.6
Ex 405:
13.6
Ex 458:
82.2
Ex 488:
94.8
Ex 514:
77.7
Ex 532:
15.2