Fluorescent Protein Properties

Excitation maxima

Excitation maxima

Emission maxima

Emission maxima

Brightness

Brightness

pKa

pKa

Bleaching time

Bleaching time

Maturation time

Maturation time
Ex max
433
Em max
475
pKa
3.2
Brightness
34.8
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
69.8
Bleaching

Percentage of excitation at

Ex 395: 39.6
Ex 405: 57.6
Ex 458: 82.7
Ex max
587
Em max
610
pKa
4.5
Brightness
15.8
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
15
Bleaching
68

Percentage of excitation at

Ex 355: 13.2
Ex 488: 7.6
Ex 514: 20.9
Ex 532: 40
Ex 543: 53.5
Ex 555: 59.9
Ex 561: 63.9
Ex 594: 86.7
Ex 612: 20.9
Ex max
589
Em max
610
pKa
3.3
Brightness
17.5
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
Bleaching

Percentage of excitation at

Ex 488: 6.8
Ex 514: 18.7
Ex 532: 37.5
Ex 543: 48.9
Ex 555: 55.5
Ex 561: 64.1
Ex 594: 90.5
Ex 612: 28.6
Ex max
516
Em max
529
pKa
Brightness
69.56
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
Bleaching

Percentage of excitation at

Ex 458: 8
Ex 488: 35.4
Ex 514: 99
Ex 532: 15
Ex max
506
Em max
518
pKa
6.5
Brightness
85
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
43.5
Bleaching
80

Percentage of excitation at

Ex 458: 22.5
Ex 488: 59.1
Ex 514: 63.3
Ex max
433
Em max
475
pKa
Brightness
13.5
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
Bleaching

Percentage of excitation at

Ex 355: 6.9
Ex 395: 33.9
Ex 405: 50.9
Ex 458: 90.8
Ex 488: 5.4
Ex max
488
Em max
507
pKa
6
Brightness
33.6
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
Bleaching

Percentage of excitation at

Ex 355: 5.6
Ex 395: 16
Ex 405: 17.3
Ex 458: 61.2
Ex 488: 99.8
Ex 514: 14.7
Ex max
487
Em max
509
pKa
6
Brightness
39.1
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
11.2
Bleaching
101

Percentage of excitation at

Ex 355: 5.6
Ex 395: 16
Ex 405: 17.3
Ex 458: 61.2
Ex 488: 99.8
Ex 514: 14.7
Ex max
487
Em max
562
pKa
4
Brightness
2
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
Bleaching
5.9

Percentage of excitation at

Ex 395: 8.6
Ex 405: 13.6
Ex 458: 82.2
Ex 488: 94.8
Ex 514: 77.7
Ex 532: 15.2
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