Fluorescent Protein Properties
Excitation maxima
Emission maxima
Brightness
pKa
Bleaching time
Maturation time
Ex max
490
Em max
508
pKa
Brightness
52.6
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 405:
5.7
Ex 458:
61.2
Ex 488:
97.6
Ex max
515
Em max
527
pKa
5.5
Brightness
66.6
Aggregation
Maturation
17.6
Bleaching
Percentage of excitation at
Ex 458:
8
Ex 488:
35.5
Ex 514:
99.1
Ex 532:
14.6
Ex max
493
Em max
509
pKa
6.5
Brightness
56
Aggregation
Maturation
Bleaching
93
Percentage of excitation at
Ex 458:
35.5
Ex 488:
91.7
Ex max
494
Em max
502
pKa
6.2
Brightness
43
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 458:
45.5
Ex 488:
86.6
Ex max
399
Em max
511
pKa
4.9
Brightness
18.6
Aggregation
Maturation
38.4
Bleaching
Percentage of excitation at
Ex 355:
35.9
Ex 395:
99.9
Ex 405:
91.8
Ex max
355
Em max
424
pKa
3.0
Brightness
3.6
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
99.7
Ex 395:
25.2
Ex 405:
4.5
Ex max
485
Em max
510
pKa
Brightness
54.1
Aggregation
Maturation
13.6
Bleaching
Percentage of excitation at
Ex 395:
12.4
Ex 405:
14.7
Ex 458:
70.9
Ex 488:
99.6
Ex 514:
20
Ex max
399
Em max
511
pKa
4.9
Brightness
26.4
Aggregation
Maturation
156.5
Bleaching
25
Percentage of excitation at
Ex 355:
39.6
Ex 395:
99.7
Ex 405:
92.6
Ex max
402
Em max
457
pKa
2.7
Brightness
32.8
Aggregation
Maturation
13
Bleaching
34
Percentage of excitation at
Ex 355:
16.2
Ex 395:
90.5
Ex 405:
94.3