Fluorescent Protein: Constitutive fluorescent proteins
Ex max
430
Em max
474
pKa
3.3
Brightness
23.1
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
7.5
Ex 395:
43.2
Ex 405:
60.7
Ex 458:
67
Ex max
395
Em max
509
pKa
4.5
Brightness
19.7
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
39.9
Ex 395:
99.9
Ex 405:
91.5
Ex 458:
26
Ex 488:
23.1
Ex max
433
Em max
475
pKa
4.7
Brightness
26.7
Aggregation
Maturation
Bleaching
36
Percentage of excitation at
Ex 355:
6.9
Ex 395:
33.9
Ex 405:
50.9
Ex 458:
90.8
Ex 488:
5.4
Ex max
456
Em max
480
pKa
Brightness
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
56.3
Ex 395:
47.5
Ex 405:
57.4
Ex 458:
99.8
Ex max
516
Em max
529
pKa
5.7
Brightness
58.5
Aggregation
Maturation
Bleaching
49
Percentage of excitation at
Ex 458:
8
Ex 488:
35.1
Ex 514:
99
Ex 532:
15
Ex max
505
Em max
515
pKa
6.2
Brightness
84.4
Aggregation
Maturation
30
Bleaching
50
Percentage of excitation at
Ex 458:
22.5
Ex 488:
59.1
Ex 514:
63.3
Ex max
497
Em max
589
pKa
5.5
Brightness
30.4
Aggregation
Maturation
15
Bleaching
111
Percentage of excitation at
Ex 395:
6.5
Ex 405:
9.8
Ex 458:
60.7
Ex 488:
95.6
Ex 514:
98.7
Ex 532:
86.8
Ex 543:
59.1
Ex 555:
26.8
Ex 561:
17.7
Ex max
435
Em max
477
pKa
5.02
Brightness
17.8
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
6.9
Ex 395:
33.9
Ex 405:
50.9
Ex 458:
90.8
Ex 488:
5.4
Ex max
558
Em max
583
pKa
Brightness
49.3
Aggregation
Maturation
1600
Bleaching
Percentage of excitation at
Ex 405:
6.7
Ex 458:
36.1
Ex 488:
46.7
Ex 514:
68.6
Ex 532:
57.7
Ex 543:
99.9
Ex 555:
85.8
Ex 561:
60.8