Fluorescent Protein: Constitutive fluorescent proteins
testing
Excitation maxima
Emission maxima
Brightness
pKa
Bleaching time
Maturation time
Ex max
554
Em max
581
pKa
4.7
Brightness
47.6
Aggregation
Maturation
60
Bleaching
64
Percentage of excitation at
Ex 355:
7.3
Ex 458:
12.8
Ex 488:
27.2
Ex 514:
54.6
Ex 532:
64.4
Ex 543:
82.2
Ex 555:
99.9
Ex 561:
91.2
Ex max
380
Em max
440
pKa
Brightness
6.3
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
72.3
Ex 395:
75.2
Ex 405:
51.8
Ex max
383
Em max
448
pKa
5.3
Brightness
18
Aggregation
Maturation
25
Bleaching
55
Percentage of excitation at
Ex 355:
42.5
Ex 395:
88.2
Ex 405:
67.2
Ex max
434
Em max
477
pKa
4.7
Brightness
13
Aggregation
Maturation
Bleaching
85
Percentage of excitation at
Ex 355:
6.9
Ex 395:
33.9
Ex 405:
50.9
Ex 458:
90.8
Ex 488:
5.4
Ex max
488
Em max
507
pKa
6
Brightness
33.6
Aggregation
Maturation
25
Bleaching
174
Percentage of excitation at
Ex 355:
5.6
Ex 395:
16
Ex 405:
17.3
Ex 458:
61.2
Ex 488:
99.8
Ex 514:
14.7
Ex max
513
Em max
527
pKa
6.9
Brightness
44.89
Aggregation
Maturation
9
Bleaching
60
Percentage of excitation at
Ex 395:
5.9
Ex 405:
5.7
Ex 458:
9.6
Ex 488:
38.1
Ex 514:
100
Ex max
580
Em max
608
pKa
4.6
Brightness
17.95
Aggregation
Maturation
130
Bleaching
150
Percentage of excitation at
Ex 488:
8.3
Ex 514:
23.8
Ex 532:
47.6
Ex 543:
57.3
Ex 555:
65.6
Ex 561:
74.9
Ex 594:
61.5
Ex 612:
12.7
Ex max
684
Em max
708
pKa
4.6
Brightness
6.4
Aggregation
Maturation
114
Bleaching
50
Percentage of excitation at
Ex 355:
44.6
Ex 395:
80.8
Ex 405:
49.6
Ex 555:
6.1
Ex 561:
7.6
Ex 594:
21.7
Ex 612:
37.1
Ex 632:
52.3
Ex 640:
56.5
Ex max
588
Em max
635
pKa
Brightness
22.1
Aggregation
Maturation
34
Bleaching
208
Percentage of excitation at
Ex 488:
8.8
Ex 514:
20.7
Ex 532:
36.4
Ex 543:
46.4
Ex 555:
54.1
Ex 561:
62.7
Ex 594:
90.7
Ex 612:
33.7