Fluorescent Protein: Constitutive fluorescent proteins
testing
Excitation maxima
Emission maxima
Brightness
pKa
Bleaching time
Maturation time
Ex max
548
Em max
561
pKa
5
Brightness
49.4
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 488:
19.3
Ex 514:
52.2
Ex 532:
57
Ex 543:
89.7
Ex 555:
82.1
Ex 561:
48.2
Ex max
460
Em max
605
pKa
2.7
Brightness
4.4
Aggregation
Maturation
150
Bleaching
Percentage of excitation at
Ex 355:
14.9
Ex 395:
34
Ex 405:
46.1
Ex 458:
100
Ex 488:
82.5
Ex 514:
39.5
Ex 532:
17.4
Ex 543:
10.6
Ex 555:
6
Ex max
437
Em max
572
pKa
5.7
Brightness
23.4
Aggregation
Maturation
138
Bleaching
Percentage of excitation at
Ex 355:
10.7
Ex 395:
54.9
Ex 405:
69.8
Ex 458:
82.5
Ex 488:
13.3
Ex max
406
Em max
526
pKa
6
Brightness
26.1
Aggregation
Maturation
1.8
Bleaching
2.8
Percentage of excitation at
Ex 355:
20.2
Ex 395:
89.5
Ex 405:
99
Ex max
568
Em max
592
pKa
6.5
Brightness
36.7
Aggregation
Maturation
30
Bleaching
4.8
Percentage of excitation at
Ex 355:
14.8
Ex 458:
5
Ex 488:
17.6
Ex 514:
38.6
Ex 532:
61.1
Ex 543:
64.4
Ex 555:
77.7
Ex 561:
89.7
Ex 594:
28
Ex max
540
Em max
553
pKa
6.7
Brightness
4.2
Aggregation
Maturation
60
Bleaching
1.4
Percentage of excitation at
Ex 458:
10.7
Ex 488:
32.4
Ex 514:
60
Ex 532:
85.8
Ex 543:
97
Ex 555:
37.3
Ex 561:
15.3
Ex max
446
Em max
611
pKa
5.6
Brightness
17.5
Aggregation
Maturation
60
Bleaching
Percentage of excitation at
Ex 355:
11.3
Ex 395:
43.2
Ex 405:
54.7
Ex 458:
91.8
Ex 488:
46.7
Ex 514:
14
Ex 532:
9.4
Ex max
604
Em max
659
pKa
5.3
Brightness
16.5
Aggregation
Maturation
27
Bleaching
730
Percentage of excitation at
Ex 488:
5.9
Ex 514:
14.5
Ex 532:
25.1
Ex 543:
36.7
Ex 555:
49.9
Ex 561:
53.5
Ex 594:
92.9
Ex 612:
85.3
Ex 632:
26.1
Ex 640:
13.1
Ex max
433
Em max
475
pKa
Brightness
16.2
Aggregation
Maturation
6.6
Bleaching
Percentage of excitation at
Ex 355:
6.9
Ex 395:
33.9
Ex 405:
50.9
Ex 458:
90.8
Ex 488:
5.4