Fluorescent Protein: Constitutive fluorescent proteins

Ex max
548
Em max
561
pKa
5
Brightness
49.4
Aggregation
Icon indicating the dimeric nature of the fluorescent protein
Maturation
Bleaching

Percentage of excitation at

Ex 488: 19.3
Ex 514: 52.2
Ex 532: 57
Ex 543: 89.7
Ex 555: 82.1
Ex 561: 48.2
Ex max
460
Em max
605
pKa
2.7
Brightness
4.4
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
150
Bleaching

Percentage of excitation at

Ex 355: 14.9
Ex 395: 34
Ex 405: 46.1
Ex 458: 100
Ex 488: 82.5
Ex 514: 39.5
Ex 532: 17.4
Ex 543: 10.6
Ex 555: 6
Ex max
437
Em max
572
pKa
5.7
Brightness
23.4
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
138
Bleaching

Percentage of excitation at

Ex 355: 10.7
Ex 395: 54.9
Ex 405: 69.8
Ex 458: 82.5
Ex 488: 13.3
Ex max
406
Em max
526
pKa
6
Brightness
26.1
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
1.8
Bleaching
2.8

Percentage of excitation at

Ex 355: 20.2
Ex 395: 89.5
Ex 405: 99
Ex max
568
Em max
592
pKa
6.5
Brightness
36.7
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
30
Bleaching
4.8

Percentage of excitation at

Ex 355: 14.8
Ex 458: 5
Ex 488: 17.6
Ex 514: 38.6
Ex 532: 61.1
Ex 543: 64.4
Ex 555: 77.7
Ex 561: 89.7
Ex 594: 28
Ex max
540
Em max
553
pKa
6.7
Brightness
4.2
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
60
Bleaching
1.4

Percentage of excitation at

Ex 458: 10.7
Ex 488: 32.4
Ex 514: 60
Ex 532: 85.8
Ex 543: 97
Ex 555: 37.3
Ex 561: 15.3
Ex max
446
Em max
611
pKa
5.6
Brightness
17.5
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
60
Bleaching

Percentage of excitation at

Ex 355: 11.3
Ex 395: 43.2
Ex 405: 54.7
Ex 458: 91.8
Ex 488: 46.7
Ex 514: 14
Ex 532: 9.4
Ex max
604
Em max
659
pKa
5.3
Brightness
16.5
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
27
Bleaching
730

Percentage of excitation at

Ex 488: 5.9
Ex 514: 14.5
Ex 532: 25.1
Ex 543: 36.7
Ex 555: 49.9
Ex 561: 53.5
Ex 594: 92.9
Ex 612: 85.3
Ex 632: 26.1
Ex 640: 13.1
Ex max
433
Em max
475
pKa
Brightness
16.2
Aggregation
Icon indicates aggregation state of the fluorescent protein
Maturation
6.6
Bleaching

Percentage of excitation at

Ex 355: 6.9
Ex 395: 33.9
Ex 405: 50.9
Ex 458: 90.8
Ex 488: 5.4
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