Fluorescent Protein: Constitutive fluorescent proteins
Ex max
433
Em max
475
pKa
3.2
Brightness
34.8
Aggregation
Maturation
69.8
Bleaching
Percentage of excitation at
Ex 395:
39.6
Ex 405:
57.6
Ex 458:
82.7
Ex max
587
Em max
610
pKa
4.5
Brightness
15.8
Aggregation
Maturation
15
Bleaching
68
Percentage of excitation at
Ex 355:
13.2
Ex 488:
7.6
Ex 514:
20.9
Ex 532:
40
Ex 543:
53.5
Ex 555:
59.9
Ex 561:
63.9
Ex 594:
86.7
Ex 612:
20.9
Ex max
589
Em max
610
pKa
3.3
Brightness
17.5
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 488:
6.8
Ex 514:
18.7
Ex 532:
37.5
Ex 543:
48.9
Ex 555:
55.5
Ex 561:
64.1
Ex 594:
90.5
Ex 612:
28.6
Ex max
516
Em max
529
pKa
Brightness
69.56
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 458:
8
Ex 488:
35.4
Ex 514:
99
Ex 532:
15
Ex max
506
Em max
518
pKa
6.5
Brightness
85
Aggregation
Maturation
43.5
Bleaching
80
Percentage of excitation at
Ex 458:
22.5
Ex 488:
59.1
Ex 514:
63.3
Ex max
433
Em max
475
pKa
Brightness
13.5
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
6.9
Ex 395:
33.9
Ex 405:
50.9
Ex 458:
90.8
Ex 488:
5.4
Ex max
488
Em max
507
pKa
6
Brightness
33.6
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 355:
5.6
Ex 395:
16
Ex 405:
17.3
Ex 458:
61.2
Ex 488:
99.8
Ex 514:
14.7
Ex max
487
Em max
509
pKa
6
Brightness
39.1
Aggregation
Maturation
11.2
Bleaching
101
Percentage of excitation at
Ex 355:
5.6
Ex 395:
16
Ex 405:
17.3
Ex 458:
61.2
Ex 488:
99.8
Ex 514:
14.7
Ex max
487
Em max
562
pKa
4
Brightness
2
Aggregation
Maturation
Bleaching
5.9
Percentage of excitation at
Ex 395:
8.6
Ex 405:
13.6
Ex 458:
82.2
Ex 488:
94.8
Ex 514:
77.7
Ex 532:
15.2