Laemmli buffer: Preparation (1x,2x & 4x) and principle

April 7, 2020 /

The Laemmli sample buffer or Laemmli buffer is used for loading and better resolving of SDS-PAGE gels. In this article, you will learn the preparation and principle of the buffer in step-by-step.

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TBE buffer (Tris-Borate-EDTA buffer) 1x, 5x & 10x

April 7, 2020 /

The TBE buffer (Tris Borate EDTA buffer) is used in DNA/RNA electrophoresis. In this article, you learn how to prepare TBE step-by-step.

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Ponceau S staining (preparation and protocol)

April 6, 2020 /

Ponceau S staining solution preparation, staining and destaining procedures were discussed in detail.

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macconkey agar hero image

MacConkey agar

March 30, 2020 /

MacConkey agar is both selective and differential media. In this article you will learn the principle and preparation of MacConkey agar.

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EMB agar with pink colonies and colonies with green sheen

EMB agar (Eosin-Methylene Blue agar)

March 29, 2020 /

The principle and preparation of EMB agar was discussed in detail. You will learn the reason behind green metallic sheen, pink colonies and blue colonies.

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Tris Buffered Saline (TBS) 10X recipe

March 10, 2020 /

Tris buffered saline (TBS) is isotonic and non-toxic. TBS provide optimal conditions for antigen – antibody interactions. TBS composed of Tris and NaCl. While the former component gives the buffering capability, the later one helps in tonicity.

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Nutrient agar and Nutrient broth

March 7, 2020 /

Nutrient agar (and broth version of it) is a general-purpose/basal medium that supports the growth of a wide range of non-fastidious organisms (microbes that can grow and thrive without specific nutritional or environmental conditions). It is commonly used for cultivation and maintenance of microbial cultures for scientific study or identification.

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Illustration depicting the principle of Sabouraud Dextrose Agar (SDA) or Sabouraud agar media.

Sabouraud dextrose agar (SDA)

March 7, 2020 /

The Sabouraud Dextrose Agar (SDA) or Sabouraud agar medium was first formulated by a French Physician Dr Raymond Jacques Adrien Sabouraud (pronounced sah-bū-rō’), in 1892 while investigating fungi which cause skin lesions.

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SOC media recipe

September 29, 2019 /

Introduction SOC (Super Optimal broth with Catabolite repression) is a variant of SOB media. SOC is a nutrient-rich culture media developed by Douglas Hanahan in 1983. SOC is typically used for bacterial transformation. Since it is nutrient-rich media, it helps bacteria to recover faster after heat shock or electroporation. Applications SOC medium is optimized for…

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YPD media

August 30, 2019 /

YPD (also known as YEPD) is a complete media used to maintain yeast culture. The media composed of Yeast extract, Peptone and Dextrose (glucose).

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Preparation of EDTA solution

August 29, 2019 /

In this article, you will learn preparation of 0.1 to 0.5M EDTA step by step. EDTA is a widely used chelating agent in biochemistry and molecular biology.

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Tris Buffer

August 12, 2019 /

Tris buffer is used in various methods ranging from DNA extraction to Gel electrophoresis. In this article you learn the preparation of Tris buffer step-by-step.

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TE Buffer

August 10, 2019 /

TE buffers or Tris-EDTA buffer is used for storage of nucleic acids. In this article, you will learn TE buffer preparation step-by-step.

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Arabidopsis Pollen Germination Medium (Li H 1999)

May 26, 2019 /

There are a handful of Arabidopsis pollen germination media. This article gives you the composition based on Li H 1999.

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TAE buffer (Tris-acetate-EDTA)

April 14, 2019 /

TAE buffer is used in electrophoresis. In this post you learn preparation of TAE buffer step-by-step.

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LB media

LB broth / LB medium

January 14, 2019 /

Giuseppe Bertani formulated LB broth in 1951 in an attempt to optimise Shigella growth and plaque formation. Although LB media is known as “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”, it was initially named as “Lysogeny Broth”. LB broth’s agar form should be designated LA but now referring as LBA/LB. There are many formulations available…

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PBST Buffer (PBS with Tween 20)

January 2, 2019 /

PBS-T is washing buffer used in many methods. Here you will learn the composition and uses of PBS-T buffer.

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4% Paraformaldehyde (PFA) solution preparation

January 1, 2019 /

Paraformaldehyde (PFA) is widely used fixative for IHC and fluorescent protein samples. in this post, you learn preparation of PFA fixative step-by-step.

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Phosphate Buffered Saline (PBS)

December 21, 2018 /

Composition and preparation of Phosphate Buffered Saline (PBS) is explained in step-by-step. PBS is used as base component for many other buffers like PBS-T, 4 % PFA etc..

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Arabidopsis Pollen Germination Medium

December 5, 2018 /

There are a handful of Pollen Germination Media (PGM) formulated for one of the widely used model plant, Arabidopsis. In this article, we have provided composition of one of the robust PGM.

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