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SOC media recipe

September 29, 2019 /

Introduction SOC (Super Optimal broth with Catabolite repression) is a variant of SOB media. SOC is a nutrient-rich culture media developed by Douglas Hanahan in 1983. SOC is typically used for bacterial transformation. Since it is nutrient-rich media, it helps bacteria to recover faster after heat shock or electroporation. Applications SOC medium is optimized for…

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YPD media

August 30, 2019 /

YPD (also known as YEPD) is a complete media used to maintain yeast culture. The media composed of Yeast extract, Peptone and Dextrose (glucose).

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Preparation of EDTA solution

August 29, 2019 /

In this article, you will learn preparation of 0.1 to 0.5M EDTA step by step. EDTA is a widely used chelating agent in biochemistry and molecular biology.

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Tris Buffer

August 12, 2019 /

Introduction: Tris (hydroxymethyl) aminomethane is also known as THAM, with pKa 8.06. Tris (M.W: 121.14) is a white crystalline powder having molecular formula C4H11NO3. It forms a clear solution in water. Tris is used as a buffering system in most of the biological experiments. Applications: Tris is a component of: DNA extraction buffer (Edwards buffer)…

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TE Buffer

August 10, 2019 /

TE buffer (a.k.a Tris EDTA buffer) is one of the extensively used buffers in preparation and storage of DNA and RNA protocols. TE buffer is also known as T10E1 Buffer due to their ratio in the working concentration, which is 10mM and 1 mM respectively. As the name suggests, the TE buffer is composed of…

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Arabidopsis Pollen Germination Medium (Li H 1999)

May 26, 2019 /

The pollen germination medium (PGM) is used for allowing the pollen to germinate either by in vitro or semi-in vitro methods. This web page deals with the composition and preparation of PGM as mentioned in the article titled “Control of Pollen Tube Tip Growth by a Rop GTPase–Dependent Pathway That Leads to Tip-Localized Calcium Influx”…

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TAE buffer (Tris-acetate-EDTA)

April 14, 2019 /

Tris-acetate-EDTA (TAE) is one of the most commonly used buffers for DNA and RNA Agarose electrophoresis. It has a lower buffering capacity compared to TBE (Tris-borate-EDTA) but runs nucleic acids faster, hence became the first choice. The composition that is currently being used is developed by the contribution of different research groups in the early…

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LB broth / LB medium

January 14, 2019 /

Giuseppe Bertani formulated LB broth in 1951 in an attempt to optimise Shigella growth and plaque formation. Although LB media is known as “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”, it was initially named as “Lysogeny Broth”. LB broth’s agar form should be designated LA but now referring as LBA/LB. There are many formulations available…

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PBST Buffer (PBS with Tween 20)

January 2, 2019 /

PBST or PBS-T (phosphate buffered saline with Tween 20) is PBS buffer with detergent such as Tween 20 or Triton X-100 (CSH Protocols recommends Tween 20). Detergent concentration can vary depends on requirement but generally vary from 0.05 – 2% (CSHP recommends 0.05). We are giving composition based on CSHL protocols in the tabular form.…

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4% Paraformaldehyde (PFA) solution preparation

January 1, 2019 /

Paraformaldehyde (PFA) in PBS is one of the widely used fixatives for Immuno-histochemistry (IHC) and fluorescent protein labelled samples. Paraformaldehyde is a polymer of formaldehyde with a wide range of monomeric units typically 8-100. PFA does not have the capacity to fix samples, hence it must be depolymerised in the solution. Heating the PFA powder…

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Phosphate Buffered Saline (PBS)

December 21, 2018 /

Phosphate buffered saline (a.k.a PBS or PBS buffer) is one of the most used buffers since it is isotonic to most of the cells. We have placed reagents required for preparation in a table. This table also contains information on molarity of each reagent and amount of salt (s) to be added for making 500mL…

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Arabidopsis Pollen Germination Medium (Leonor 2007)

December 5, 2018 /

There are a handful of Pollen Germination Media (PGM) formulated for one of the widely used model plant, Arabidopsis. In this article, we have provided composition of one of the robust PGM. This PGM composition is adopted from a publication by Leonor 2007 (see the reference at the end of the article). If you are…

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