Enumeration of fungi is possible on DRBC media. The image shows growth of Rhizopus spp. as distinct countable units on DRBC media. On the other-hand on PDA, it is spread all over the plate.

DRBC agar: Principle and preparation

June 6, 2022 / Comments Off on DRBC agar: Principle and preparation

Dichloran-rose bengal chloramphenicol agar or DRBC agar or DRBC media is used to enumerate microbes that spread, mostly fungi. In this article, we have discussed history, principle, and the media’s preparation.

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2x YT medium

February 19, 2022 / Comments Off on 2x YT medium

Introduction 2xYT is a nutritionally rich liquid microbial growth medium used for propagation of recombinant strains of Escherichia coli and M13 bacteriophage or other filamentous single-stranded DNA bacteriophages. Applications This medium contains a mainly enzymatic digest of casein and yeast extract. Casein enzymic hydrolysate acts as a source of nitrogenous compounds like amino acids and…

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Sodium acetate buffer

February 18, 2022 / Comments Off on Sodium acetate buffer

Introduction Various authors (Gomori, 1955; Pearse 1980) are cited as a source for buffer composition, although it should be said that probably the first author specifying this buffer was Walpole (1914). However, the composition of the cited buffers is really similar. Thanks to the pH variability and composition of available compounds, it is sometimes the first…

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MS media (Murashige – Skoog) composition and preparation

February 18, 2022 / Comments Off on MS media (Murashige – Skoog) composition and preparation

Introduction The composition of the growing medium is crucial in every experiment containing living organisms. The need for a well-balanced plant medium arose at the beginning of hydroponic cultivation and plant tissue cultures. The first recipes for plant tissue cultures are connected with great names of plant in vitro – Hildebrandt and others (e.g. Hildebrandt…

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Citrate Buffer recipe

February 13, 2022 / Comments Off on Citrate Buffer recipe

Introduction The authorship is often contributed to Gomori (1955) but Gomori is in this publication citing an article that is seven years older (Lillie 1948) as original source. Citrate buffer is a universal buffer used in various reactions. It is sometimes combined with sodium citrate or completely replaced by sodium citrate buffer. However, alkalized citrate…

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Terrific Broth

December 20, 2021 / Comments Off on Terrific Broth

The terrific broth is used as a superior alternative to LB media for the high yield of plasmids and recombinant proteins.

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Illustration showing E.coli growing in minimal media by davis

Minimal media (Davis formulation)

September 13, 2020 / Comments Off on Minimal media (Davis formulation)

Minimal media is a defined media i.e., media with known composition. In this article, we have described preparation of the minimal media formulated by Davis step by step.

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steps involved in potato dextrose agar preparation

Potato Dextrose Agar (PDA)

September 5, 2020 / Comments Off on Potato Dextrose Agar (PDA)

Learn to prepare Potato dextrose agar media step-by-step. PDA is one of the most used media for cultivation and maintenance of fungi.

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Laemmli buffer: Preparation (1x,2x & 4x) and principle

April 7, 2020 / Comments Off on Laemmli buffer: Preparation (1x,2x & 4x) and principle

The Laemmli sample buffer or Laemmli buffer is used for loading and better resolving of SDS-PAGE gels. In this article, you will learn the preparation and principle of the buffer in step-by-step.

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TBE buffer (Tris-Borate-EDTA buffer) 1x, 5x & 10x

April 7, 2020 / Comments Off on TBE buffer (Tris-Borate-EDTA buffer) 1x, 5x & 10x

The TBE buffer (Tris Borate EDTA buffer) is used in DNA/RNA electrophoresis. In this article, you learn how to prepare TBE step-by-step.

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Ponceau S staining (preparation and protocol)

April 6, 2020 / Comments Off on Ponceau S staining (preparation and protocol)

Ponceau S staining solution preparation, staining and destaining procedures were discussed in detail.

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macconkey agar hero image

MacConkey agar

March 30, 2020 / Comments Off on MacConkey agar

MacConkey agar is both selective and differential media. In this article you will learn the principle and preparation of MacConkey agar.

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EMB agar with pink colonies and colonies with green sheen

EMB agar (Eosin-Methylene Blue agar)

March 29, 2020 / Comments Off on EMB agar (Eosin-Methylene Blue agar)

The principle and preparation of EMB agar was discussed in detail. You will learn the reason behind green metallic sheen, pink colonies and blue colonies.

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Tris Buffered Saline (TBS) 10X recipe

March 10, 2020 / Comments Off on Tris Buffered Saline (TBS) 10X recipe

Tris buffered saline (TBS) is isotonic and non-toxic. TBS provide optimal conditions for antigen – antibody interactions. TBS composed of Tris and NaCl. While the former component gives the buffering capability, the later one helps in tonicity.

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Nutrient agar and Nutrient broth

March 7, 2020 / Comments Off on Nutrient agar and Nutrient broth

Nutrient agar (and broth version of it) is a general-purpose/basal medium that supports the growth of a wide range of non-fastidious organisms (microbes that can grow and thrive without specific nutritional or environmental conditions). It is commonly used for cultivation and maintenance of microbial cultures for scientific study or identification.

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Illustration depicting the principle of Sabouraud Dextrose Agar (SDA) or Sabouraud agar media.

Sabouraud dextrose agar (SDA)

March 7, 2020 / Comments Off on Sabouraud dextrose agar (SDA)

The Sabouraud Dextrose Agar (SDA) or Sabouraud agar medium was first formulated by a French Physician Dr Raymond Jacques Adrien Sabouraud (pronounced sah-bū-rō’), in 1892 while investigating fungi which cause skin lesions.

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SOC media recipe

September 29, 2019 / Comments Off on SOC media recipe

Introduction SOC (Super Optimal broth with Catabolite repression) is a variant of SOB media. SOC is a nutrient-rich culture media developed by Douglas Hanahan in 1983. SOC is typically used for bacterial transformation. Since it is nutrient-rich media, it helps bacteria to recover faster after heat shock or electroporation. Applications SOC medium is optimized for…

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YPD media

August 30, 2019 / Comments Off on YPD media

YPD (also known as YEPD) is a complete media used to maintain yeast culture. The media composed of Yeast extract, Peptone and Dextrose (glucose).

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Preparation of EDTA solution

August 29, 2019 / Comments Off on Preparation of EDTA solution

In this article, you will learn preparation of 0.1 to 0.5M EDTA step by step. EDTA is a widely used chelating agent in biochemistry and molecular biology.

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Tris Buffer

August 12, 2019 / Comments Off on Tris Buffer

Tris buffer is used in various methods ranging from DNA extraction to Gel electrophoresis. In this article you learn the preparation of Tris buffer step-by-step.

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TE Buffer

August 10, 2019 / Comments Off on TE Buffer

TE buffers or Tris-EDTA buffer is used for storage of nucleic acids. In this article, you will learn TE buffer preparation step-by-step.

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Arabidopsis Pollen Germination Medium (Li H 1999)

May 26, 2019 / Comments Off on Arabidopsis Pollen Germination Medium (Li H 1999)

There are a handful of Arabidopsis pollen germination media. This article gives you the composition based on Li H 1999.

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TAE buffer (Tris-acetate-EDTA)

April 14, 2019 / Comments Off on TAE buffer (Tris-acetate-EDTA)

TAE buffer is used in electrophoresis. In this post you learn preparation of TAE buffer step-by-step.

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LB media

LB broth / LB medium

January 14, 2019 / Comments Off on LB broth / LB medium

Giuseppe Bertani formulated LB broth in 1951 in an attempt to optimise Shigella growth and plaque formation. Although LB media is known as “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”, it was initially named as “Lysogeny Broth”. LB broth’s agar form should be designated LA but now referring as LBA/LB. There are many formulations available…

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PBST Buffer (PBS with Tween 20)

January 2, 2019 / Comments Off on PBST Buffer (PBS with Tween 20)

PBS-T is washing buffer used in many methods. Here you will learn the composition and uses of PBS-T buffer.

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