Buffers and solutions

Laemmli buffer: Preparation (1x,2x & 4x) and principle

The Laemmli sample buffer or Laemmli buffer is used for loading and better resolving of SDS-PAGE gels. In this article, you will learn the preparation and principle of the buffer in step-by-step.

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TBE buffer (Tris-Borate-EDTA buffer) 1x, 5x & 10x

The TBE buffer (Tris Borate EDTA buffer) is used in DNA/RNA electrophoresis. In this article, you learn how to prepare TBE step-by-step.

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Ponceau S staining (preparation and protocol)

Ponceau S staining solution preparation, staining and destaining procedures were discussed in detail.

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Tris Buffered Saline (TBS) 10X recipe

Tris buffered saline (TBS) is isotonic and non-toxic. TBS provide optimal conditions for antigen – antibody interactions. TBS composed of Tris and NaCl. While the former component gives the buffering capability, the later one helps in tonicity.

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Preparation of EDTA solution

In this article, you will learn preparation of 0.1 to 0.5M EDTA step by step. EDTA is a widely used chelating agent in biochemistry and molecular biology.

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Tris Buffer

Tris buffer is used in various methods ranging from DNA extraction to Gel electrophoresis. In this article you learn the preparation of Tris buffer step-by-step.

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TE Buffer

TE buffers or Tris-EDTA buffer is used for storage of nucleic acids. In this article, you will learn TE buffer preparation step-by-step.

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TAE buffer (Tris-acetate-EDTA)

TAE buffer is used in electrophoresis. In this post you learn preparation of TAE buffer step-by-step.

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PBST Buffer (PBS with Tween 20)

PBS-T is washing buffer used in many methods. Here you will learn the composition and uses of PBS-T buffer.

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4% Paraformaldehyde (PFA) solution preparation

Paraformaldehyde (PFA) is widely used fixative for IHC and fluorescent protein samples. in this post, you learn preparation of PFA fixative step-by-step.

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Phosphate Buffered Saline (PBS)

Composition and preparation of Phosphate Buffered Saline (PBS) is explained in step-by-step. PBS is used as base component for many other buffers like PBS-T, 4 % PFA etc..

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