Culture media

macconkey agar hero image

MacConkey agar

MacConkey agar is both selective and differential media. In this article you will learn the principle and preparation of MacConkey agar.

Read More
EMB agar with pink colonies and colonies with green sheen

EMB agar (Eosin-Methylene Blue agar)

The principle and preparation of EMB agar was discussed in detail. You will learn the reason behind green metallic sheen, pink colonies and blue colonies.

Read More

Nutrient agar and Nutrient broth

Nutrient agar (and broth version of it) is a general-purpose/basal medium that supports the growth of a wide range of non-fastidious organisms (microbes that can grow and thrive without specific nutritional or environmental conditions). It is commonly used for cultivation and maintenance of microbial cultures for scientific study or identification.

Read More
Illustration depicting the principle of Sabouraud Dextrose Agar (SDA) or Sabouraud agar media.

Sabouraud dextrose agar (SDA)

The Sabouraud Dextrose Agar (SDA) or Sabouraud agar medium was first formulated by a French Physician Dr Raymond Jacques Adrien Sabouraud (pronounced sah-bū-rō’), in 1892 while investigating fungi which cause skin lesions.

Read More

SOC media recipe

Introduction SOC (Super Optimal broth with Catabolite repression) is a variant of SOB media. SOC is a nutrient-rich culture media developed by Douglas Hanahan in 1983. SOC is typically used for bacterial transformation. Since it is nutrient-rich media, it helps bacteria to recover faster after heat shock or electroporation. Applications SOC medium is optimized for…

Read More

YPD media

YPD (also known as YEPD) is a complete media used to maintain yeast culture. The media composed of Yeast extract, Peptone and Dextrose (glucose).

Read More

Arabidopsis Pollen Germination Medium (Li H 1999)

There are a handful of Arabidopsis pollen germination media. This article gives you the composition based on Li H 1999.

Read More
LB media

LB broth / LB medium

Giuseppe Bertani formulated LB broth in 1951 in an attempt to optimise Shigella growth and plaque formation. Although LB media is known as “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”, it was initially named as “Lysogeny Broth”. LB broth’s agar form should be designated LA but now referring as LBA/LB. There are many formulations available…

Read More

Arabidopsis Pollen Germination Medium

There are a handful of Pollen Germination Media (PGM) formulated for one of the widely used model plant, Arabidopsis. In this article, we have provided composition of one of the robust PGM.

Read More