Culture media

Illustration showing E.coli growing in minimal media by davis

Minimal media (Davis formulation)

Minimal media is a defined media i.e., media with known composition. In this article, we have described preparation of the minimal media formulated by Davis step by step.

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steps involved in potato dextrose agar preparation

Potato Dextrose Agar (PDA)

Learn to prepare Potato dextrose agar media step-by-step. PDA is one of the most used media for cultivation and maintenance of fungi.

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macconkey agar hero image

MacConkey agar

MacConkey agar is both selective and differential media. In this article you will learn the principle and preparation of MacConkey agar.

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EMB agar with pink colonies and colonies with green sheen

EMB agar (Eosin-Methylene Blue agar)

The principle and preparation of EMB agar was discussed in detail. You will learn the reason behind green metallic sheen, pink colonies and blue colonies.

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Nutrient agar and Nutrient broth

Nutrient agar (and broth version of it) is a general-purpose/basal medium that supports the growth of a wide range of non-fastidious organisms (microbes that can grow and thrive without specific nutritional or environmental conditions). It is commonly used for cultivation and maintenance of microbial cultures for scientific study or identification.

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Illustration depicting the principle of Sabouraud Dextrose Agar (SDA) or Sabouraud agar media.

Sabouraud dextrose agar (SDA)

The Sabouraud Dextrose Agar (SDA) or Sabouraud agar medium was first formulated by a French Physician Dr Raymond Jacques Adrien Sabouraud (pronounced sah-bū-rō’), in 1892 while investigating fungi which cause skin lesions.

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SOC media recipe

Introduction SOC (Super Optimal broth with Catabolite repression) is a variant of SOB media. SOC is a nutrient-rich culture media developed by Douglas Hanahan in 1983. SOC is typically used for bacterial transformation. Since it is nutrient-rich media, it helps bacteria to recover faster after heat shock or electroporation. Applications SOC medium is optimized for…

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YPD media

YPD (also known as YEPD) is a complete media used to maintain yeast culture. The media composed of Yeast extract, Peptone and Dextrose (glucose).

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Arabidopsis Pollen Germination Medium (Li H 1999)

There are a handful of Arabidopsis pollen germination media. This article gives you the composition based on Li H 1999.

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LB media

LB broth / LB medium

Giuseppe Bertani formulated LB broth in 1951 in an attempt to optimise Shigella growth and plaque formation. Although LB media is known as “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”, it was initially named as “Lysogeny Broth”. LB broth’s agar form should be designated LA but now referring as LBA/LB. There are many formulations available…

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Arabidopsis Pollen Germination Medium

There are a handful of Pollen Germination Media (PGM) formulated for one of the widely used model plant, Arabidopsis. In this article, we have provided composition of one of the robust PGM.

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