Culture media

Enumeration of fungi is possible on DRBC media. The image shows growth of Rhizopus spp. as distinct countable units on DRBC media. On the other-hand on PDA, it is spread all over the plate.

DRBC agar: Principle and preparation

Dichloran-rose bengal chloramphenicol agar or DRBC agar or DRBC media is used to enumerate microbes that spread, mostly fungi. In this article, we have discussed history, principle, and the media’s preparation.

2x YT medium

Introduction 2xYT is a nutritionally rich liquid microbial growth medium used for propagation of recombinant strains of Escherichia coli and M13 bacteriophage or other filamentous single-stranded DNA bacteriophages. Applications This medium contains a mainly enzymatic digest of casein and yeast extract. Casein enzymic hydrolysate acts as a source of nitrogenous compounds like amino acids and…

MS media (Murashige – Skoog) composition and preparation

Introduction The composition of the growing medium is crucial in every experiment containing living organisms. The need for a well-balanced plant medium arose at the beginning of hydroponic cultivation and plant tissue cultures. The first recipes for plant tissue cultures are connected with great names of plant in vitro – Hildebrandt and others (e.g. Hildebrandt…

Terrific Broth

The terrific broth is used as a superior alternative to LB media for the high yield of plasmids and recombinant proteins.

Illustration showing E.coli growing in minimal media by davis

Minimal media (Davis formulation)

Minimal media is a defined media i.e., media with known composition. In this article, we have described preparation of the minimal media formulated by Davis step by step.

steps involved in potato dextrose agar preparation

Potato Dextrose Agar (PDA)

Learn to prepare Potato dextrose agar media step-by-step. PDA is one of the most used media for cultivation and maintenance of fungi.

MacConkey agar

MacConkey agar is both selective and differential media. In this article you will learn the principle and preparation of MacConkey agar.

EMB agar with pink colonies and colonies with green sheen

EMB agar (Eosin-Methylene Blue agar)

The principle and preparation of EMB agar was discussed in detail. You will learn the reason behind green metallic sheen, pink colonies and blue colonies.

Nutrient agar and Nutrient broth

Nutrient agar (and broth version of it) is a general-purpose/basal medium that supports the growth of a wide range of non-fastidious organisms (microbes that can grow and thrive without specific nutritional or environmental conditions). It is commonly used for cultivation and maintenance of microbial cultures for scientific study or identification.

Sabouraud dextrose agar (SDA)

The Sabouraud Dextrose Agar (SDA) or Sabouraud agar medium was first formulated by a French Physician Dr Raymond Jacques Adrien Sabouraud (pronounced sah-bū-rō’), in 1892 while investigating fungi which cause skin lesions.

SOC media recipe

Introduction SOC (Super Optimal broth with Catabolite repression) is a variant of SOB media. SOC is a nutrient-rich culture media developed by Douglas Hanahan in 1983. SOC is typically used for bacterial transformation. Since it is nutrient-rich media, it helps bacteria to recover faster after heat shock or electroporation. Applications SOC medium is optimized for…

YPD media

YPD (also known as YEPD) is a complete media used to maintain yeast culture. The media composed of Yeast extract, Peptone and Dextrose (glucose).

Arabidopsis Pollen Germination Medium (Li H 1999)

There are a handful of Arabidopsis pollen germination media. This article gives you the composition based on Li H 1999.

LB broth / LB medium

Giuseppe Bertani formulated LB broth in 1951 in an attempt to optimise Shigella growth and plaque formation. Although LB media is known as “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”, it was initially named as “Lysogeny Broth”. LB broth’s agar form should be designated LA but now referring as LBA/LB. There are many formulations available…

Arabidopsis Pollen Germination Medium

There are a handful of Pollen Germination Media (PGM) formulated for one of the widely used model plant, Arabidopsis. In this article, we have provided composition of one of the robust PGM.

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