Culture media
Dichloran-rose bengal chloramphenicol agar or DRBC agar or DRBC media is used to enumerate microbes that spread, mostly fungi. In this article, we have discussed history, principle, and the media’s preparation.
Read MoreIntroduction 2xYT is a nutritionally rich liquid microbial growth medium used for propagation of recombinant strains of Escherichia coli and M13 bacteriophage or other filamentous single-stranded DNA bacteriophages. Applications This medium contains a mainly enzymatic digest of casein and yeast extract. Casein enzymic hydrolysate acts as a source of nitrogenous compounds like amino acids and…
Read MoreIntroduction The composition of the growing medium is crucial in every experiment containing living organisms. The need for a well-balanced plant medium arose at the beginning of hydroponic cultivation and plant tissue cultures. The first recipes for plant tissue cultures are connected with great names of plant in vitro – Hildebrandt and others (e.g. Hildebrandt…
Read MoreThe terrific broth is used as a superior alternative to LB media for the high yield of plasmids and recombinant proteins.
Read MoreMinimal media is a defined media i.e., media with known composition. In this article, we have described preparation of the minimal media formulated by Davis step by step.
Read MoreLearn to prepare Potato dextrose agar media step-by-step. PDA is one of the most used media for cultivation and maintenance of fungi.
Read MoreMacConkey agar is both selective and differential media. In this article you will learn the principle and preparation of MacConkey agar.
Read MoreThe principle and preparation of EMB agar was discussed in detail. You will learn the reason behind green metallic sheen, pink colonies and blue colonies.
Read MoreNutrient agar (and broth version of it) is a general-purpose/basal medium that supports the growth of a wide range of non-fastidious organisms (microbes that can grow and thrive without specific nutritional or environmental conditions). It is commonly used for cultivation and maintenance of microbial cultures for scientific study or identification.
Read MoreThe Sabouraud Dextrose Agar (SDA) or Sabouraud agar medium was first formulated by a French Physician Dr Raymond Jacques Adrien Sabouraud (pronounced sah-bū-rō’), in 1892 while investigating fungi which cause skin lesions.
Read MoreIntroduction SOC (Super Optimal broth with Catabolite repression) is a variant of SOB media. SOC is a nutrient-rich culture media developed by Douglas Hanahan in 1983. SOC is typically used for bacterial transformation. Since it is nutrient-rich media, it helps bacteria to recover faster after heat shock or electroporation. Applications SOC medium is optimized for…
Read MoreThere are a handful of Arabidopsis pollen germination media. This article gives you the composition based on Li H 1999.
Read MoreGiuseppe Bertani formulated LB broth in 1951 in an attempt to optimise Shigella growth and plaque formation. Although LB media is known as “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”, it was initially named as “Lysogeny Broth”. LB broth’s agar form should be designated LA but now referring as LBA/LB. There are many formulations available…
Read MoreThere are a handful of Pollen Germination Media (PGM) formulated for one of the widely used model plant, Arabidopsis. In this article, we have provided composition of one of the robust PGM.
Read More