Fluorescent Protein Properties
Excitation maxima
Emission maxima
Brightness
pKa
Bleaching time
Maturation time
Ex max
588
Em max
633
pKa
5.4
Brightness
25
Aggregation
Maturation
20
Bleaching
84
Percentage of excitation at
Ex 355:
6.5
Ex 488:
7.1
Ex 514:
16.5
Ex 532:
30.6
Ex 543:
42.7
Ex 555:
49.8
Ex 561:
55.2
Ex 594:
95.7
Ex 612:
37.4
Ex 632:
5.6
Ex max
440
Em max
620
pKa
6.5
Brightness
3.4
Aggregation
Maturation
180
Bleaching
Percentage of excitation at
Ex 355:
15
Ex 395:
56
Ex 405:
71.1
Ex 458:
86.4
Ex 488:
26.3
Ex 514:
6.9
Ex 532:
7.1
Ex 543:
8.3
Ex 555:
9.7
Ex max
548
Em max
559
pKa
5
Brightness
31
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 488:
17.8
Ex 514:
52.2
Ex 532:
58.8
Ex 543:
92.5
Ex 555:
76.2
Ex 561:
41.3
Ex max
551
Em max
565
pKa
5.5
Brightness
39.6
Aggregation
Maturation
108
Bleaching
5
Percentage of excitation at
Ex 355:
5
Ex 488:
16.7
Ex 514:
52.7
Ex 532:
56.2
Ex 543:
81.1
Ex 555:
95.9
Ex 561:
70.5
Ex max
609
Em max
657
pKa
6.2
Brightness
8.8
Aggregation
Maturation
20
Bleaching
178
Percentage of excitation at
Ex 355:
12.2
Ex 395:
5.3
Ex 514:
12.9
Ex 532:
22.6
Ex 543:
30.6
Ex 555:
41.8
Ex 561:
47.1
Ex 594:
79.8
Ex 612:
98.2
Ex 632:
37.1
Ex 640:
19.6
Ex max
506
Em max
517
pKa
5.7
Brightness
92.8
Aggregation
Maturation
10
Bleaching
158
Percentage of excitation at
Ex 458:
21.8
Ex 488:
61.5
Ex 514:
59.3
Ex max
600
Em max
650
pKa
5.4
Brightness
13.4
Aggregation
Maturation
35
Bleaching
255
Percentage of excitation at
Ex 488:
8.7
Ex 514:
20.2
Ex 532:
34
Ex 543:
47.4
Ex 555:
59.6
Ex 561:
63.3
Ex 594:
98.7
Ex 612:
79.2
Ex 632:
20.6
Ex 640:
9.7
Ex max
548
Em max
562
pKa
6.5
Brightness
49
Aggregation
Maturation
150
Bleaching
6.4
Percentage of excitation at
Ex 458:
5.4
Ex 488:
19.6
Ex 514:
50.6
Ex 532:
61.7
Ex 543:
92.5
Ex 555:
80.6
Ex 561:
47.1
Ex max
549
Em max
565
pKa
6.5
Brightness
34.8
Aggregation
Maturation
270
Bleaching
228
Percentage of excitation at
Ex 458:
5.6
Ex 488:
20
Ex 514:
51.9
Ex 532:
63.6
Ex 543:
91.8
Ex 555:
85
Ex 561:
53.2