Fluorescent Protein Properties
Excitation maxima
Emission maxima
Brightness
pKa
Bleaching time
Maturation time
Ex max
551
Em max
592
pKa
4.8
Brightness
14.8
Aggregation
Maturation
264
Bleaching
368
Percentage of excitation at
Ex 458:
10.9
Ex 488:
29.4
Ex 514:
61.4
Ex 532:
73.3
Ex 543:
89.5
Ex 555:
98.9
Ex 561:
87
Ex 594:
5.6
Ex max
569
Em max
593
pKa
5.4
Brightness
56.2
Aggregation
Maturation
36
Bleaching
190
Percentage of excitation at
Ex 488:
11.5
Ex 514:
26.7
Ex 532:
48.7
Ex 543:
50.5
Ex 555:
65.3
Ex 561:
81.9
Ex 594:
17.3
Ex max
597
Em max
633
pKa
Brightness
7.6
Aggregation
Maturation
Bleaching
1002
Percentage of excitation at
Ex 355:
24.9
Ex 395:
10.1
Ex 405:
9
Ex 458:
11.4
Ex 488:
11.6
Ex 514:
20.4
Ex 532:
32.1
Ex 543:
44.5
Ex 555:
56.2
Ex 561:
59.4
Ex 594:
100
Ex 612:
58.8
Ex 632:
17
Ex 640:
10.7
Ex max
574
Em max
596
pKa
4.5
Brightness
26.1
Aggregation
Maturation
50
Bleaching
15
Percentage of excitation at
Ex 355:
11.2
Ex 488:
11.9
Ex 514:
30.7
Ex 532:
55.8
Ex 543:
63.5
Ex 555:
73.4
Ex 561:
81.3
Ex 594:
37
Ex max
399
Em max
454
pKa
2.7
Brightness
32.4
Aggregation
Maturation
12
Bleaching
53
Percentage of excitation at
Ex 355:
16.2
Ex 395:
92.2
Ex 405:
95.9
Ex max
568
Em max
585
pKa
5.7
Brightness
11.4
Aggregation
Maturation
Bleaching
5.1
Percentage of excitation at
Ex 355:
10.7
Ex 488:
13.2
Ex 514:
32.7
Ex 532:
56.4
Ex 543:
63.3
Ex 555:
78.9
Ex 561:
91
Ex 594:
18
Ex max
462
Em max
492
pKa
4.3
Brightness
54
Aggregation
Maturation
Bleaching
163
Percentage of excitation at
Ex 395:
15.2
Ex 405:
24.8
Ex 458:
94.5
Ex 488:
10.2
Ex max
434
Em max
474
pKa
4.5
Brightness
25.2
Aggregation
Maturation
112.2
Bleaching
Percentage of excitation at
Ex 355:
6
Ex 395:
34.4
Ex 405:
52.5
Ex 458:
86
Ex max
434
Em max
474
pKa
3.1
Brightness
27.9
Aggregation
Maturation
33.5
Bleaching
90
Percentage of excitation at
Ex 355:
5.9
Ex 395:
32
Ex 405:
49.7
Ex 458:
89.8