Fluorescent Protein Properties
Excitation maxima
Emission maxima
Brightness
pKa
Bleaching time
Maturation time
Ex max
458
Em max
480
pKa
4.7
Brightness
21
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 395:
23.2
Ex 405:
36.8
Ex 458:
99.9
Ex max
483
Em max
506
pKa
4.7
Brightness
33.9
Aggregation
Maturation
11
Bleaching
Percentage of excitation at
Ex 395:
5
Ex 405:
7.9
Ex 458:
69.1
Ex 488:
95.2
Ex 514:
6.2
Ex max
555
Em max
584
pKa
3.8
Brightness
48
Aggregation
Maturation
100
Bleaching
48
Percentage of excitation at
Ex 355:
5.2
Ex 488:
10.7
Ex 514:
34.9
Ex 532:
48.8
Ex 543:
72.2
Ex 555:
99.8
Ex 561:
87.1
Ex max
555
Em max
584
pKa
4.6
Brightness
33.2
Aggregation
Maturation
100
Bleaching
337
Percentage of excitation at
Ex 355:
5.5
Ex 458:
5.2
Ex 488:
11.6
Ex 514:
34.3
Ex 532:
47.5
Ex 543:
69.9
Ex 555:
100
Ex 561:
90.3
Ex max
508
Em max
524
pKa
5.5
Brightness
31
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 458:
18
Ex 488:
55.3
Ex 514:
90.2
Ex 532:
10.4
Ex max
554
Em max
581
pKa
4.7
Brightness
95.22
Aggregation
Maturation
60
Bleaching
70
Percentage of excitation at
Ex 355:
7.3
Ex 458:
12.8
Ex 488:
27.2
Ex 514:
54.6
Ex 532:
64.4
Ex 543:
82.2
Ex 555:
99.9
Ex 561:
91.2
Ex max
514
Em max
527
pKa
Brightness
56.7
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 458:
11.1
Ex 488:
42.2
Ex 514:
100
Ex 532:
19.6
Ex max
490
Em max
508
pKa
Brightness
46.4
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 405:
5.7
Ex 458:
61.2
Ex 488:
97.6
Ex max
515
Em max
528
pKa
6
Brightness
52.5
Aggregation
Maturation
17.6
Bleaching
15
Percentage of excitation at
Ex 355:
4.9
Ex 395:
5.9
Ex 405:
5.7
Ex 458:
9.6
Ex 488:
38.1
Ex 514:
100