Fluorescent Protein Properties
Excitation maxima
Emission maxima
Brightness
pKa
Bleaching time
Maturation time
Ex max
530
Em max
541
pKa
6.8
Brightness
35.7
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 458:
10.8
Ex 488:
38.5
Ex 514:
59
Ex 532:
98.1
Ex 543:
41.1
Ex 555:
8.1
Ex max
590
Em max
649
pKa
4.5
Brightness
4.1
Aggregation
Maturation
100
Bleaching
53
Percentage of excitation at
Ex 488:
7.2
Ex 514:
20.4
Ex 532:
39.7
Ex 543:
54
Ex 555:
63.3
Ex 561:
67.5
Ex 594:
96.1
Ex 612:
45.4
Ex max
598
Em max
625
pKa
Brightness
12.9
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 488:
5.5
Ex 514:
15.8
Ex 532:
28.8
Ex 543:
42.8
Ex 555:
56.7
Ex 561:
59
Ex 594:
95.6
Ex 612:
65.6
Ex max
584
Em max
607
pKa
4.5
Brightness
12.5
Aggregation
Maturation
60
Bleaching
6.2
Percentage of excitation at
Ex 488:
8.4
Ex 514:
22.6
Ex 532:
43.6
Ex 543:
55.5
Ex 555:
61
Ex 561:
66.2
Ex 594:
74
Ex 612:
14.8
Ex max
590
Em max
612
pKa
Brightness
Aggregation
Maturation
Bleaching
Percentage of excitation at
Ex 488:
5.9
Ex 514:
18
Ex 532:
35.3
Ex 543:
50.1
Ex 555:
58.3
Ex 561:
61
Ex 594:
96.8
Ex 612:
31.9
Ex max
558
Em max
605
pKa
4.4
Brightness
39.2
Aggregation
Maturation
168
Bleaching
Percentage of excitation at
Ex 488:
9.2
Ex 514:
31
Ex 532:
48.3
Ex 543:
66.2
Ex 555:
97
Ex 561:
99.1
Ex 594:
12.4
Ex max
559
Em max
600
pKa
5.3
Brightness
43
Aggregation
Maturation
150
Bleaching
123
Percentage of excitation at
Ex 355:
6.4
Ex 488:
10.7
Ex 514:
33.8
Ex 532:
49.6
Ex 543:
66.5
Ex 555:
97.1
Ex 561:
98.6
Ex 594:
10.6
Ex max
558
Em max
592
pKa
4.8
Brightness
57.6
Aggregation
Maturation
136.5
Bleaching
349
Percentage of excitation at
Ex 488:
9.9
Ex 514:
30.7
Ex 532:
46.3
Ex 543:
63.5
Ex 555:
97.5
Ex 561:
96.9
Ex 594:
9.7
Ex max
569
Em max
594
pKa
5.3
Brightness
70
Aggregation
Maturation
174
Bleaching
277
Percentage of excitation at
Ex 488:
14.9
Ex 514:
27.7
Ex 532:
48.6
Ex 543:
50.7
Ex 555:
66.7
Ex 561:
81.8
Ex 594:
18