YPD media

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    Introduction

    YPD media (yeast extract peptone dextrose) also known as YEPD (yeast extract peptone dextrose) named after its composition. YPD media is a complete media formulated by Sherman F. Yeast can grow in media containing salts and glucose. However, the presence of peptone and yeast extract aids faster growth.

    Applications

    Used as complete media. Not useful for selection of auxotroph.

    pH

    pH is not adjusted for this medium. However, in case of requirement, adjust pH to 7.0 with 5M NaOH (~200µL for 1L).

    Composition

     

    Reagent% of reagent (W/V)Composition for 500 mlComposition for 1L
    Yeast extract1%5g10g
    peptone2%10g20g
    Dextrose (Glucose)2%10g20g
    Bacto Agar (optional)1.5 - 2%  

     

    Reagents

    • Yeast Extract
    • Peptone
    • Dextrose (Glucose)
    • Bacto agar
    • Deionized water

    Instruments and other requirements

    • Autoclave
    • Flasks
    • Petri dishes (if preparing agar plates)
    • Magnetic stirrer and pellet

    Procedure

    1. Suspend the ingredients in deionized water.
    2. Heat if necessary to dissolve the medium completely.
    3. Sterilize by filtration (0.22 micron) or autoclaving (15 minutes at 121 °C).

    Storage

    Store for several weeks at room temperature.

    Caution

    Peptone and yeast extract are hygroscopic. Close the bottle caps tightly.

    Reference

     

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