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YPD media

Introduction

YPD media (yeast extract peptone dextrose) also known as YEPD (yeast extract peptone dextrose) named after it’s composition. YPD media is a complete media formulated by Sherman F. Yeast can grow in media containing salts and glucose. However, the presence of peptone and yeast extract aids faster growth.

Applications

Used as complete media. Not useful for selection of auxotroph.

pH

pH is not adjusted for this medium. However, in case of requirement, adjust pH to 7.0 with 5M NaOH (~200µL for 1L).

Composition

pH is not adjusted for this medium. However, in case of requirement, adjust pH to 7.0 with 5M NaOH (~200µL).
pH is not adjusted for this medium. However, in case of requirement, adjust pH to 7.0 with 5M NaOH (~200µL).
Reagent % of reagent (W/V) Composition for 500 ml Composition for 1L
Yeast extract 1% 5g 10g
peptone 2% 10g 20g
Dextrose (Glucose) 2% 10g 20g
Bacto Agar (optional) 1.5 - 2%

Click here to get molarity and dilution calculator for making stocks

Reagents

  • Yeast Extract
  • Peptone
  • Dextrose (Glucose)
  • Bacto agar
  • Deionized water

Instruments and other requirements

  • Autoclave
  • Flasks
  • Petri dishes (if preparing agar plates)
  • Magnetic stirrer and pellet

Preparation

  1. Suspend the ingredients in deionize3d water.
  2. Heat if necessary to dissolve the medium completely.
  3. Sterilize by filtration (0.22 micron) or autoclaving (15 minutes at 121 °C).

Caution

Peptone and yeast extract are hygroscopic. Close the bottle caps tightly.

Storage

Store for several weeks at room temperature.

References

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