Culture Media
2x YT medium
Table Of Contents Introduction 2xYT medium is a nutritionally rich liquid formulation that is used for the propagation of recombinant…
Arabidopsis Pollen Germination Medium
There are a handful of Pollen Germination Media (PGM) formulated for one of the widely used model plant, Arabidopsis. In…
Arabidopsis Pollen Germination Medium (Li H 1999)
There are a handful of Arabidopsis pollen germination media. This article gives you the composition based on Li H 1999.
DRBC agar: Principle and preparation
Dichloran-rose bengal chloramphenicol agar or DRBC agar or DRBC media is used to enumerate microbes that spread, mostly fungi. In…
EMB agar (Eosin-Methylene Blue agar)
The principle and preparation of EMB agar was discussed in detail. You will learn the reason behind green metallic sheen,…
LB broth / LB medium
Giuseppe Bertani formulated LB broth in 1951 in an attempt to optimise Shigella growth and plaque formation. Although LB media…
MacConkey agar
MacConkey agar is both selective and differential media. In this article you will learn the principle and preparation of MacConkey…
Minimal media (Davis formulation)
Minimal media is a defined media i.e., media with known composition. In this article, we have described preparation of the…
MS media (Murashige – Skoog) composition and preparation
Table Of Contents Introduction The composition of the growing medium is crucial in every experiment containing living organisms. The need…
Nutrient agar and Nutrient broth
Nutrient agar (and broth version of it) is a general-purpose/basal medium that supports the growth of a wide range of…