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SOC media recipe

By sharebiology | September 29, 2019 |

Introduction SOC (Super Optimal broth with Catabolite repression) is a variant of SOB media. SOC is a nutrient-rich culture media developed by Douglas Hanahan in 1983. SOC is typically used for bacterial transformation. Since it is nutrient-rich media, it helps bacteria to recover faster after heat shock or electroporation. Applications SOC medium is optimized for…

YPD media

By sharebiology | August 30, 2019 |

YPD (also known as YEPD) is a complete media used to maintain yeast culture. The media composed of Yeast extract, Peptone and Dextrose (glucose).

Preparation of EDTA solution

By sharebiology | August 29, 2019 |

In this article, you will learn preparation of 0.1 to 0.5M EDTA step by step. EDTA is a widely used chelating agent in biochemistry and molecular biology.

Tris Buffer

By sharebiology | August 12, 2019 |

Tris buffer is used in various methods ranging from DNA extraction to Gel electrophoresis. In this article you learn the preparation of Tris buffer step-by-step.

TE Buffer

By sharebiology | August 10, 2019 |

TE buffers or Tris-EDTA buffer is used for storage of nucleic acids. In this article, you will learn TE buffer preparation step-by-step.

Arabidopsis Pollen Germination Medium (Li H 1999)

By sharebiology | May 26, 2019 |

There are a handful of Arabidopsis pollen germination media. This article gives you the composition based on Li H 1999.

tissue clearing methods for plants and animals

Tissue clearing methods – Articles summary

By sharebiology | May 3, 2019 |

The tissue clearing methods that are listed below are compatible with fluorescent proteins. With these methods, you can image fluorescent samples 100s of microns deep that are otherwise difficult to image even 20-50 microns deep.

TAE buffer (Tris-acetate-EDTA)

By sharebiology | April 14, 2019 |

TAE buffer is used in electrophoresis. In this post you learn preparation of TAE buffer step-by-step.

LB broth / LB medium

By sharebiology | January 14, 2019 |

Giuseppe Bertani formulated LB broth in 1951 in an attempt to optimise Shigella growth and plaque formation. Although LB media is known as “Luria Broth”, “Luria-Bertani” medium, or “Lennox Broth”, it was initially named as “Lysogeny Broth”. LB broth’s agar form should be designated LA but now referring as LBA/LB. There are many formulations available…

PBST Buffer (PBS with Tween 20)

By sharebiology | January 2, 2019 |

PBS-T is washing buffer used in many methods. Here you will learn the composition and uses of PBS-T buffer.

4% Paraformaldehyde (PFA) solution preparation

By sharebiology | January 1, 2019 |

Paraformaldehyde (PFA) is widely used fixative for IHC and fluorescent protein samples. in this post, you learn preparation of PFA fixative step-by-step.

Phosphate Buffered Saline (PBS)

By sharebiology | December 21, 2018 |

Composition and preparation of Phosphate Buffered Saline (PBS) is explained in step-by-step. PBS is used as base component for many other buffers like PBS-T, 4 % PFA etc..

Arabidopsis Pollen Germination Medium

By sharebiology | December 5, 2018 |

There are a handful of Pollen Germination Media (PGM) formulated for one of the widely used model plant, Arabidopsis. In this article, we have provided composition of one of the robust PGM.