Fluorescent proteins

Illustration showing the AtPCNA1 expression pattern in plant cells

AtPCNA1-An ‘S’ phase progression marker for plants

AtPCNA1-sGFP under native promoter was used to track the progression of the S-phase in plants. This article describes the advantages,…

Read More
Blunt end cloning hero image showing an overview of steps involved in the cloning

Blunt-end cloning: Ultimate guide

Blunt-end cloning is a universal cloning method. In this article, you will learn history, applications, advantages, limitations, and cloning procedure…

Read More
Blunt-end TOPO cloning hero image

Blunt-end TOPO Cloning

Blunt-end TOPO cloning is a better alternative to the conventional (ligation-based) cloning method. This article discusses the principle, advantages, and…

Read More

Cytrap (Cell Cycle Tracking in Plant Cells)

CyTrap is a dual-color fluorescent cell cycle marker system to mark S-G2 (red) and G2-M (green) developed in Arabidopsis.

Read More
Directional TOPO cloning hero image

Directional TOPO Cloning

Directional TOPO cloning is an easy sub-cloning method to clone DNA fragments without ligase. This article discusses the principle, advantages,…

Read More
Gibson assembly hero image

Gibson assembly

Gibson assembly is one of the most used methods for seamless cloning. In this article, you will learn history, applications,…

Read More
Gibson vs In Fusion hero image

Gibson assembly vs. In-Fusion cloning

Gibson assembly and In-Fusion are the two most used cloning methods for scar-free cloning of multiple DNA fragments. This article…

Read More
hero image for In-Fusion cloning post

In-Fusion Cloning

In-Fusion cloning is one of the most used methods for seamless cloning. In this article, you will learn history, applications,…

Read More
Plant cell cycle indicator title image

PlaCCI (Plant Cell Cycle Indicator)

PlaCCI (Plant Cell Cycle Indicator) is a relatively new cell cycle marking system in plants that is similar to the…

Read More
Primer designing hero image

Primer Designing – Demonstration step by step

Primer designing is the first and one of the critical steps for successful cloning. In this article, we have demonstrated…

Read More
TA cloning hero image

TA cloning: Ultimate guide

TA cloning is a universal cloning method. In this article, you will learn history, applications, advantages, limitations, and cloning procedure…

Read More
TOPO TA cloning hero image

TOPO TA Cloning

TOPO TA cloning is a better alternative to the conventional (ligation-based) TA cloning method. This article discusses the principle, advantages,…

Read More
Share via